Vector is a carrier molecule that will be inserted with DNA fragment with desired gene transform into host.
Normally, a vector will have these common characteristics : 1. Origin of replication 2. Cloning site (one or multiple restriction site) 3. Selectable marker 4. Antibiotic resistance gene 5. Promoter along with regulator control
Some of the examples of vector that commonly used to transfer the DNA vector into host are : 1. Plasmid : isolated from bacteria, covalently closed circular structure 2. virus : incorporate DNA fragment into viruses which will then be transfer to host by infection properties of bacteria 3. Cosmid : genetically modified vector that consist of cos site of virus and plasmid DNA 4. Phagemid : a phage whose genome contains a plasmid DNA 5. Ti plasmid : a vector that used transfer into plant cell (host), originate from Agrobacterium tumefacien 6. Bacterial Artificial chromosome (BAC) plasmid (or F-plasmid) which use for transforming and cloning in bacteria 7. Yeast Artificial Chromosome (YAC) : using yeast chromosome as vector, capable of carrying large inserts of DNA from 200 to 2000 kb
The restriction enzyme that use to generate DNA fragment will be used to cut vector. This will produce a compatible end which enable the DNA fragment to be inserted into vector.