History Highlight on Discovery of Gene Cloning
- 1952-53 : Genetic demonstration of how phage DNA being cleave by restriction enzyme in bacteria
- 1961 : Genetic recombination demonstrated
- 1967 : First DNA ligase was isolated
- 1968 : Isolation of first restriction factor that could selectively cut phage DNA
- 1970 : Isolation of Restriction enzyme that selectively cut
- 1972 : First DNA recombinant and transformation of first E.coli
- 1974 : NEB open business
- 1975 : Launch of REBASE (Restriction Enzyme Database)
- 1962-1977 : Introduction of Maxam-Gilbert and Sanger Sequencing
- 1977 : First cloning vector pBR322
- 1978 : Nobel Prize awarded to Smith Arber and Nathans that for discovery of restriction enzyme
- 1983 : Introduction of PCR
- 1985 : Generation of pUC plasmid
- 1987 : First automated sequencer available
- 1991 : Introduction of User Cloning
- 2000 : in vitro synthesis of whole genome
- 2007 : Introduction of Ligation-independent cloning
- 2008 : Synthesis of Mycoplasm genitalium genome using Gibson Assembly and Introduction of HF restriction enzyme
- 2010 : First synthetic life Mycoplasma mycoides