Antibiotic ScreeningThe introduction of recombinant DNA into bacteria is not efficient. So, it is important to determine the host cells which carry the recombinant DNA. The cells that do not have taken up the recombinant DNA are killed selectively as the recombinant DNA usually contain selectable marker gene which usually is the antibiotic resistance gene encoded by vector are enable survive. Selectable marker often is antibiotic resistant genes. The bacteria are test by ability to growth on medium plate that contain the antibiotic and the bacterial colonies that can survive and multiple on that plate means the it successfully taken up the recombinant vector and expressed the antibiotic resistance gene. The antibiotics resistance encoding gene normally are ampicillin, chloroamphenicol, tetracyclic and etc.
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